micropulser electroporation apparatus (Bio-Rad)
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micropulser electroporation apparatus
Micropulser Electroporation Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1652 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/micropulser+electroporation+apparatus/MicroPulser+Electroporator/pmc12809317-174-19-22
Average 96 stars, based on 1652 article reviews
Micropulser Electroporation Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1652 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/micropulser+electroporation+apparatus/MicroPulser+Electroporator/pmc12809317-174-19-22
Average 96 stars, based on 1652 article reviews
micropulser electroporation apparatus - by Bioz Stars,
2026-09
96/100 stars
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Plasmid Preparation:Article Title: Urine- and serum-based ELISA using a new recombinant chimeric protein to diagnose tegumentary and visceral leishmaniasis: a preliminary study. Article Snippet: Laboratory diagnosis of leishmaniasis is hampered by the variable sensitivity and/or specificity of the tests.. In the present study, we developed a new recombinant antigen based on a chimeric protein, called CHIMISA, which was composed of specific B-cell epitopes from Leishmania antigenic proteins recently identified in an immunoproteomics approach using sera samples from visceral leishmaniasis (VL) and VL/HIV co-infected patients.. The protein was produced containing specific B-cell epitopes from four parasite proteins (SUZ41772.1, SUZ41881.1, AYU79515.1, and SUZ44007.1) and used as an antigen in ELISA with serum and urine samples for diagnosing VL, tegumentary leishmaniasis (TL), and VL/HIV co-infection. Article Title: Development of two recombinant vaccines against Clostridioides difficile infection and immunogenicity in pregnant sows and neonatal piglets. Article Snippet: Introduction: Clostridioides difficile is the main cause of antibiotic-associated diarrhea in humans and is a major enteropathogen in several animal species.. In newborn piglets, colonic lesions caused by C. difficile A and B toxins (TcdA and TcdB, respectively) cause diarrhea and significant production losses.. Objective: The present study aimed to develop two recombinant vaccines from immunogenic C-terminal fragments of TcdA and TcdB and evaluate the immune response in rabbits and in breeding sows. Article Title: Evaluation of a serum and urine-based ELISA testing distinct antigens for the diagnosis of tegumentary leishmaniasis. Article Snippet: The diagnosis of tegumentary leishmaniasis (TL) is hampered by variable sensitivity and/or specificity of the tests.. In this context, the identification of more refined antigens could contribute to improve the diagnostic quality, as well as the employ of less invasive biological samples.. In this study, the eukaryotic initiation factor 5a (eIF5A) protein, one specific B-cell epitope predicted in the protein sequence, and a parasite antigenic preparation (SLA) were evaluated as antigens in ELISA experiments, which were reacted against paired serum and urine samples from 170 patients. Article Title: A Multiepitope Protein Based on Selected Synthetic Peptides Enables Effective Serological Screening of Exposure to Mycobacterium leprae Article Snippet: .. Electrocompetent Escherichia coli C43 cells (Phoneutria, Brazil) were transformed by electroporation using a 1.8 kV electric pulse with a Article Title: A Gifsy prophage-encoded protein confers broad phage resistance in Salmonella enterica and is widely distributed across Enterobacteriaceae . Article Snippet: .. Electrocompetent S. LT2 and E. coli K-12 strains were transformed with empty vector pWKS30, pWKS30-GiPD474, and pWKS30-Remain using Article Title: A Gifsy prophage-encoded protein confers broad phage resistance in Salmonella enterica and is widely distributed across Enterobacteriaceae Article Snippet: .. Electrocompetent S . LT2 and E. coli K-12 strains were transformed with empty vector pWKS30, pWKS30-GiPD474, and pWKS30-Remain using Electroporation:Article Title: Urine- and serum-based ELISA using a new recombinant chimeric protein to diagnose tegumentary and visceral leishmaniasis: a preliminary study. Article Snippet: Laboratory diagnosis of leishmaniasis is hampered by the variable sensitivity and/or specificity of the tests.. In the present study, we developed a new recombinant antigen based on a chimeric protein, called CHIMISA, which was composed of specific B-cell epitopes from Leishmania antigenic proteins recently identified in an immunoproteomics approach using sera samples from visceral leishmaniasis (VL) and VL/HIV co-infected patients.. The protein was produced containing specific B-cell epitopes from four parasite proteins (SUZ41772.1, SUZ41881.1, AYU79515.1, and SUZ44007.1) and used as an antigen in ELISA with serum and urine samples for diagnosing VL, tegumentary leishmaniasis (TL), and VL/HIV co-infection. Article Title: Decoding the impact of interspecies interactions on biofilm matrix components Article Snippet: .. Electroporation was performed using pre-chilled 1 mm gap electroporation cuvettes (BIO-RAD) and a Article Title: Development of two recombinant vaccines against Clostridioides difficile infection and immunogenicity in pregnant sows and neonatal piglets. Article Snippet: Introduction: Clostridioides difficile is the main cause of antibiotic-associated diarrhea in humans and is a major enteropathogen in several animal species.. In newborn piglets, colonic lesions caused by C. difficile A and B toxins (TcdA and TcdB, respectively) cause diarrhea and significant production losses.. Objective: The present study aimed to develop two recombinant vaccines from immunogenic C-terminal fragments of TcdA and TcdB and evaluate the immune response in rabbits and in breeding sows. Article Title: Evaluation of a serum and urine-based ELISA testing distinct antigens for the diagnosis of tegumentary leishmaniasis. Article Snippet: The diagnosis of tegumentary leishmaniasis (TL) is hampered by variable sensitivity and/or specificity of the tests.. In this context, the identification of more refined antigens could contribute to improve the diagnostic quality, as well as the employ of less invasive biological samples.. In this study, the eukaryotic initiation factor 5a (eIF5A) protein, one specific B-cell epitope predicted in the protein sequence, and a parasite antigenic preparation (SLA) were evaluated as antigens in ELISA experiments, which were reacted against paired serum and urine samples from 170 patients. Article Title: A Multiepitope Protein Based on Selected Synthetic Peptides Enables Effective Serological Screening of Exposure to Mycobacterium leprae Article Snippet: .. Electrocompetent Escherichia coli C43 cells (Phoneutria, Brazil) were transformed by electroporation using a 1.8 kV electric pulse with a Article Title: A Gifsy prophage-encoded protein confers broad phage resistance in Salmonella enterica and is widely distributed across Enterobacteriaceae . Article Snippet: .. Electrocompetent S. LT2 and E. coli K-12 strains were transformed with empty vector pWKS30, pWKS30-GiPD474, and pWKS30-Remain using Article Title: An intron-split microRNA mediates cleavage of the mRNA encoded by low phosphate root in Solanaceae Article Snippet: .. The three level 2 constructs carried by E. coli DH5α bacteria were transferred into Agrobacterium tumefaciens GV3101 (pMP90) by electroporation using a Article Title: A Gifsy prophage-encoded protein confers broad phage resistance in Salmonella enterica and is widely distributed across Enterobacteriaceae Article Snippet: .. Electrocompetent S . LT2 and E. coli K-12 strains were transformed with empty vector pWKS30, pWKS30-GiPD474, and pWKS30-Remain using Clone Assay:Article Title: Development of two recombinant vaccines against Clostridioides difficile infection and immunogenicity in pregnant sows and neonatal piglets. Article Snippet: Introduction: Clostridioides difficile is the main cause of antibiotic-associated diarrhea in humans and is a major enteropathogen in several animal species.. In newborn piglets, colonic lesions caused by C. difficile A and B toxins (TcdA and TcdB, respectively) cause diarrhea and significant production losses.. Objective: The present study aimed to develop two recombinant vaccines from immunogenic C-terminal fragments of TcdA and TcdB and evaluate the immune response in rabbits and in breeding sows. Expressing:Article Title: Development of two recombinant vaccines against Clostridioides difficile infection and immunogenicity in pregnant sows and neonatal piglets. Article Snippet: Introduction: Clostridioides difficile is the main cause of antibiotic-associated diarrhea in humans and is a major enteropathogen in several animal species.. In newborn piglets, colonic lesions caused by C. difficile A and B toxins (TcdA and TcdB, respectively) cause diarrhea and significant production losses.. Objective: The present study aimed to develop two recombinant vaccines from immunogenic C-terminal fragments of TcdA and TcdB and evaluate the immune response in rabbits and in breeding sows. Article Title: Evaluation of a serum and urine-based ELISA testing distinct antigens for the diagnosis of tegumentary leishmaniasis. Article Snippet: The diagnosis of tegumentary leishmaniasis (TL) is hampered by variable sensitivity and/or specificity of the tests.. In this context, the identification of more refined antigens could contribute to improve the diagnostic quality, as well as the employ of less invasive biological samples.. In this study, the eukaryotic initiation factor 5a (eIF5A) protein, one specific B-cell epitope predicted in the protein sequence, and a parasite antigenic preparation (SLA) were evaluated as antigens in ELISA experiments, which were reacted against paired serum and urine samples from 170 patients. Electroporation Bacterial Transformation:Article Title: Development of two recombinant vaccines against Clostridioides difficile infection and immunogenicity in pregnant sows and neonatal piglets. Article Snippet: Introduction: Clostridioides difficile is the main cause of antibiotic-associated diarrhea in humans and is a major enteropathogen in several animal species.. In newborn piglets, colonic lesions caused by C. difficile A and B toxins (TcdA and TcdB, respectively) cause diarrhea and significant production losses.. Objective: The present study aimed to develop two recombinant vaccines from immunogenic C-terminal fragments of TcdA and TcdB and evaluate the immune response in rabbits and in breeding sows. Purification:Article Title: Evaluation of a serum and urine-based ELISA testing distinct antigens for the diagnosis of tegumentary leishmaniasis. Article Snippet: The diagnosis of tegumentary leishmaniasis (TL) is hampered by variable sensitivity and/or specificity of the tests.. In this context, the identification of more refined antigens could contribute to improve the diagnostic quality, as well as the employ of less invasive biological samples.. In this study, the eukaryotic initiation factor 5a (eIF5A) protein, one specific B-cell epitope predicted in the protein sequence, and a parasite antigenic preparation (SLA) were evaluated as antigens in ELISA experiments, which were reacted against paired serum and urine samples from 170 patients. Transformation Assay:Article Title: A Multiepitope Protein Based on Selected Synthetic Peptides Enables Effective Serological Screening of Exposure to Mycobacterium leprae Article Snippet: .. Electrocompetent Escherichia coli C43 cells (Phoneutria, Brazil) were transformed by electroporation using a 1.8 kV electric pulse with a Article Title: A Gifsy prophage-encoded protein confers broad phage resistance in Salmonella enterica and is widely distributed across Enterobacteriaceae . Article Snippet: .. Electrocompetent S. LT2 and E. coli K-12 strains were transformed with empty vector pWKS30, pWKS30-GiPD474, and pWKS30-Remain using Article Title: A Gifsy prophage-encoded protein confers broad phage resistance in Salmonella enterica and is widely distributed across Enterobacteriaceae Article Snippet: .. Electrocompetent S . LT2 and E. coli K-12 strains were transformed with empty vector pWKS30, pWKS30-GiPD474, and pWKS30-Remain using Recombinant:Article Title: A Multiepitope Protein Based on Selected Synthetic Peptides Enables Effective Serological Screening of Exposure to Mycobacterium leprae Article Snippet: .. Electrocompetent Escherichia coli C43 cells (Phoneutria, Brazil) were transformed by electroporation using a 1.8 kV electric pulse with a Construct:Article Title: An intron-split microRNA mediates cleavage of the mRNA encoded by low phosphate root in Solanaceae Article Snippet: .. The three level 2 constructs carried by E. coli DH5α bacteria were transferred into Agrobacterium tumefaciens GV3101 (pMP90) by electroporation using a Bacteria:Article Title: An intron-split microRNA mediates cleavage of the mRNA encoded by low phosphate root in Solanaceae Article Snippet: .. The three level 2 constructs carried by E. coli DH5α bacteria were transferred into Agrobacterium tumefaciens GV3101 (pMP90) by electroporation using a |